Analysis and Simulation of Steady States in Metabolic Pathways with Petri Nets

Computer assisted analysis and simulation of biochemical pathways can improve the understanding of the structure and the dynamics of these systems considerably. The construction and quantitative analysis of kinetic models is often impeded by the lack of reliable data. However, as the topological structure of biochemical systems can be regarded to remain constant in time, a qualitative analysis of a pathway model was shown to be quite promising as it can render a lot of useful knowledge, e.g., about its structural invariants. This paper deals with pathways whose substances have reached a dynamic concentration equilibrium (steady state). It is argued that appreciated tools from biochemistry and also low-level Petri nets can yield only part of the desired results, whereas executable high-level net models lead to a number of valuable additional insights by combining symbolic analysis and simulation.